Multimeric protected fluorescent reagents

US10787573B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Frank Zheng · Fremont, US
• Jeremiah Hanes · Woodside, US
• Gene Shen · Santa Clara, US
• Louis Brogley · Santa Cruz, US
• Stephen Yue · Eugene, US
• Yuri Lapin · Newark, US
• John Lyle · Fremont, US
• Honey Osuna · San Francisco, US
• Andrei G. Fedorov · Atlanta, US
• Lubomir Sebo · Redwood City, US

Key dates

• Filing date: Dec 4, 2018
• Grant date: Sep 29, 2020
• Expiry date: Dec 4, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/533
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.