Bradyrhizobium monooxygenase and use thereof for preparation of chiral sulfoxide
US10787651B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Apr 13, 2018 |
| Grant date | Sep 29, 2020 |
| Priority date | — |
| Expiry date | Apr 13, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.