Methods for efficient intracellular delivery using anisotropic magnetic particles

US10787657B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Pei-Yu E. Chiou · Los Angeles, US
• Michael A. Teitell · Calabasas, US
• Ming-Yu Lin · Bakersfield, US
• Yi-Chien Wu · Taichung, TW
• Jessica Zhou · Chino, US

Key dates

• Filing date: Dec 21, 2016
• Grant date: Sep 29, 2020
• Expiry date: Oct 6, 2037

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B82Y5/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In various embodiments methods are provided for delivering an agent of interest (e.g., protein, antibody, nucleic acid) into cells. In certain embodiments the method comprises contacting the cells with anisotropic magnetic particles in the presence of the agent; and applying a substantially uniform magnetic field to said magnetic particles where movement of said particles induced by said magnetic field introduces transient openings into said cell facilitating entry of said agent of interest into said cells.