Field-effect apparatus and methods for sequencing nucleic acids

US10787704B2 · kind B2 · utility

Assignees

• Illumina, Inc. · US
• The Regents of the University of California · US

Inventors

• Kevin L. Gunderson · San Diego, US
• Jingwei Bai · Beijing, CN
• Cheng-Yao Chen · San Diego, US
• Jeffrey G. Mandell · Village of La Jolla, US
• Sergio Peisajovich · San Diego, US
• Philip G. Collins · Oakland, US
• Gregory A. Weiss · Irvine, US
• Boyan Boyanov · Portland, US

Key dates

• Filing date: May 11, 2016
• Grant date: Sep 29, 2020
• Expiry date: Nov 8, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure provides a method for sequencing nucleic acids. The method can include polymerase catalyzed incorporation of nucleotides into a nascent nucleic acid strand against a nucleic acid template, wherein the polymerase is attached to a charge sensor that detects nucleotide incorporation events. One or more non-natural nucleotide types that each produce a unique signatures at the charge sensor can be used to uniquely identify different nucleotides in the template nucleic acid.