Flow cytometry with dual laser beams
US10788411B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 2017 |
| Grant date | Sep 29, 2020 |
| Priority date | — |
| Expiry date | Apr 21, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1438
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A system, method, and apparatus are provided for cytometry with dual laser beams. In one example, the method includes directing an incident light beam from a source to enter an optical waveplate; polarizing the incident light beam into a polarized light beam in response to the incident light beam entering through the optical waveplate; directing the polarized light beam to enter a birefringent crystal; separating the polarized light beam into an ordinary light beam and an extraordinary light beam in response to the polarized light beam entering the birefringent crystal; directing the ordinary light beam and the extraordinary light beam to enter a lens; focusing the ordinary light beam and the extraordinary light beam into dual light beams separated by a beam displacement; and coupling the dual light beams to form a sample region having substantially uniform light intensity to analyze moving particles in the particle analyzer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.