Identifying organisms in a sample using sequencing kinetic signatures

US10793903B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Benjamin Flusberg · Atlanta, US
• Stephen Turner · Menlo Park, US
• Jon Sorenson · Alameda, US
• Kevin Travers · Menlo Park, US
• Cheryl Heiner · La Honda, US

Key dates

• Filing date: Sep 28, 2016
• Grant date: Oct 6, 2020
• Expiry date: Jan 19, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/154
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid.