Methods for simultaneous amplification of target loci

US10793912B2 · kind B2 · utility

Assignee

• Natera, Inc. · US

Inventors

• Joshua Babiarz · Castro Valley, US
• Tudor Popiliu Constantin · Chino Hills, US
• Lane A. Eubank · Redwood City, US
• George Gemelos · Walnut, US
• Matthew Hill · Belmont, US
• Huseyin Eser Kirkizlar · Los Angeles, US
• Matthew Rabinowitz · San Francisco, US
• Onur Sakarya · Redwood City, US
• Styrmir Sigurjonsson · San Jose, US
• Bernhard Zimmermann · Manteca, US

Key dates

• Filing date: Apr 30, 2019
• Grant date: Oct 6, 2020
• Expiry date: Apr 30, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.