Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples
US10794900B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2017 |
| Grant date | Oct 6, 2020 |
| Priority date | — |
| Expiry date | Dec 8, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/8557
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.