Immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B1 and aflatoxin B1, and complex affinity column
US10794912B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 7, 2018 |
| Grant date | Oct 6, 2020 |
| Priority date | — |
| Expiry date | Aug 25, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/385
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An immunoadsorbent for purifying fumonisin B1, aflatoxin B1, ochratoxin A, zearalenone and sterigmatocystin; a complex affinity column and its preparation method; and a method for detecting the mycotoxins using the same are provided. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B1 monoclonal antibody, an anti-aflatoxin B1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11. The complex affinity column can be used for purification and detection of a fumonisin B1 sample, an aflatoxin B1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.