Spatial genomics with co-registered histology
US10796781B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 15, 2016 |
| Grant date | Oct 6, 2020 |
| Priority date | — |
| Expiry date | May 14, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/367
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Three dimensional tumor volume is analyzed spatially by genomics and transcriptomics, or both. Thin sections are cut with a microtome using a tape transfer technique so that the thin tissue sections remain without any distortion or deformation, in sequence relative to their position in the original tissue, so that a three-dimensional coordinate system can be anchored to each tissue section. Small samples of tissue are extracted or punched from very precise locations in the supports using the coordinate system as a grid, with known x,y,z location, and subjected to genomic sequencing. Sequence data is analyzed using the three dimensional coordinate system, so that the original tissue is thereby analyzed as a three dimensional system volume. At the same time, alternate tissue sections (or the same sections from which samples have been extracted with sample punches) can be subjected to staining and imaging to do histochemical analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.