Patent · US Active

Droplet-based linked-fragment sequencing

US10801059B2 · kind B2 · utility

6Cited by
42References
21Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 28, 2017
Grant dateOct 13, 2020
Priority date—
Expiry dateJul 3, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2600/166
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention generally relates to sequencing library preparation methods. In certain embodiments, two or more template nucleic acids are joined together by a linking molecule, such as a PEG derivative. Identical copies of a nucleic acid fragment or both strands of a duplex fragment may be linked together. The linked nucleic acids are amplified, creating linked amplicons. Emulsion PCR with linked primers creates linked template nucleic acids for seeding sequencing clusters and errors can be readily identified by their presence on only one of the linked fragments.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.