Gas-phase purification for accurate isobaric tag-based quantification

US10809267B2 · kind B2 · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• Joshua J. Coon · Charlottesville, US
• Michael S. Westphall · Fitchburg, US

Key dates

• Filing date: Jun 1, 2017
• Grant date: Oct 20, 2020
• Expiry date: Jul 9, 2037

Classification

• Technology area (CPC —): General

Abstract

Described herein are mass spectrometry systems and methods which improve the accuracy of isobaric tag-based quantification by alleviating the pervasive problem of precursor interference and co-isolation of impurities through gas-phase purification. During the gas-phase purification, the mass-to-charge ratios of precursor ions within at least a selected range are selectively changed allowing ions having similar unmodified mass-to-charge ratios to be separated before further isolation, fragmentation or analysis.