Fermentation methods for producing steviol glycosides using high pH and compositions obtained therefrom

US10815513B2 · kind B2 · utility

Assignees

• CARGILL, INCORPORATED · US
• EVOLVA SA · CH

Inventors

• James C. Anderson · Eagle, US
• Manuel Quiros Asensio · Jægersborg, DK
• Simon Carlsen · Copenhagen, DK
• Ting Liu Carlson · Dayton, US
• Veronique Douchin · Copenhagen, DK
• Ariene M. Fosmer · Eden Prairie, US
• Hans Peter Smits · Paseo Los Muchachos, DK

Key dates

• Filing date: May 27, 2016
• Grant date: Oct 27, 2020
• Expiry date: May 27, 2036

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A23V2002/00
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Disclosed are methods for producing steviol glycosides, such as rebaudioside D and rebaudioside M, using engineered yeast. In some embodiments, the methods include fermenting with a yeast at a high pH such as of about 5.8 or greater. In some embodiments, the methods can be carried out by first growing the yeast at a lower first pH, and then adjusting the pH to a higher pH.