Patent · US Active

Methods and systems for processing polynucleotides

US10815525B2 · kind B2 · utility

73Cited by
267References
48Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 12, 2019
Grant dateOct 27, 2020
Priority date
Expiry dateJun 12, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2563/179
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.