Cell line screening method
US10816548B2 · kind B2 · utility
0Cited by
5References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 19, 2018 |
| Grant date | Oct 27, 2020 |
| Priority date | — |
| Expiry date | Jul 12, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/14
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a novel cell line development method useful to screen for recombinant protein production. The method utilizes a membrane-anchored reporter or an intracellular reporter residing in the expression vector for a gene of interest to facilitate initial cell selection by FACS or MACS. A switching mechanism can be used to delete the reporter from the chromosome by providing an appropriate DNA recombinase, which turns the selected cells into production cells that secrete the protein of interest without co-expression of the reporter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.