Production of thapsigargins by thapsia cell suspension culture

US10822628B2 · kind B2 · utility

Assignee

• Phyton Holdings, LLC · US

Inventors

• Gilbert Gorr · Glückstadt, DE
• Harald Heckenmuller · Seevetal, DE
• David Alexander Ullisch · Hamburg, DE
• Jens Stefan Wilke · Hamburg, DE
• Yantree Devi Sankar-Thomas · Tornesch, DE

Key dates

• Filing date: Dec 2, 2014
• Grant date: Nov 3, 2020
• Expiry date: Dec 2, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P5/007
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of producing sesquiterpene lactones of the thapsigargin family, the method comprising the steps of: (a) culturing plant cells of the genus Thapsia in a nutrient medium in a suspension cell culture, wherein the cells produce one or more sesquiterpene lactones of the thapsigargin family; and (b) recovering one or more sesquiterpene lactones of the thapsigargin family produced in (a). The present invention further relates to a suspension cell culture comprising plant cells of the genus Thapsia, wherein the plant cells are capable of producing one or more sesquiterpene lactones of the thapsigargin family and to a plant cell biomass comprising plant cells of the genus Thapsia obtained from said suspension cell culture.