Probe set for analyzing a DNA sample and method for using the same

US10822640B2 · kind B2 · utility

Assignee

• VANADIS DIAGNOSTICS · SE

Inventors

• Carl Oscar Fredrik Dahl · Sigtuna, SE
• Olof John Ericsson · Uppsala, SE
• Filip Karlsson · Solna, SE
• Fredrik Roos · Stuvsta-Snättringe, SE

Key dates

• Filing date: Nov 8, 2019
• Grant date: Nov 3, 2020
• Expiry date: Nov 8, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6858
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure provides, inter alia, a probe system probe system for analyzing a nucleic acid sample. In some embodiments, the probe system may comprise: a set of identifier oligonucleotides of sequence B, a set of splint oligonucleotides of formula X′-A′-B′-Z′, wherein sequence A′ is complementary to a genomic fragment and sequence B′ is complementary to at least one member of the set of identifier oligonucleotides, and one or more probe sequences comprising X and Z. Each splint oligonucleotide is capable of hybridizing to the probe sequences, a member of the set of identifier oligonucleotides and a genomic fragment, thereby producing a ligatable complex of formula X-A-B-Z. The probe system can be used to identify a chromosome aneuploidy in cell free DNA, for example.