Hybrid multi-step nucleic acid amplification
US10822648B1 · kind B1 · utility
1Cited by
4References
5Claims
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Key dates
| Filing date | Jul 31, 2017 |
| Grant date | Nov 3, 2020 |
| Priority date | — |
| Expiry date | Nov 8, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method.
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