Patent · US Active

Methods for single-stranded nucleic acid library preparation

US10822651B2 · kind B2 · utility

0Cited by
1References
32Claims
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Inventors

Key dates

Filing dateOct 27, 2017
Grant dateNov 3, 2020
Priority date—
Expiry dateOct 27, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2563/179
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Aspects of the invention relate to methods and compositions for preparing and analyzing a single-stranded sequencing library from a double-stranded DNA (e.g., double-stranded cfDNA) sample. In some embodiments, the sample includes double-stranded DNA (dsDNA) molecules, and damaged dsDNA (e.g., nicked dsDNA) molecules. In some embodiments, the sample includes single-stranded DNA (ssDNA) molecules. The subject methods facilitate the collection of information, including strand-pairing and connectivity information, from dsDNA, ssDNA and damaged DNA (e.g., nicked DNA) molecules in a sample, thereby providing enhanced diagnostic information as compared to sequencing libraries that are prepared using conventional methods.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.