Method for multi-color fluorescence imaging under single exposure, imaging method and imaging system
US10823945B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 6, 2017 |
| Grant date | Nov 3, 2020 |
| Priority date | — |
| Expiry date | Oct 22, 2038 |
Classification
- Technology area (CPC H)Electricity
- CPC primaryH04N13/257
- WIPO fieldAudio-visual technology
- WIPO sectorElectrical engineering
Abstract
The present disclosure relates to a method for multi-color fluorescence imaging under a single exposure, an imaging method and system. The imaging system includes: a fluorescence microscope, configured to obtain a real image of the sample; a spatial mask, disposed behind the fluorescence microscope, and configured to perform mask modulation on the real image of the sample; a 4f system, disposed behind the spatial mask, in which the real image of the sample passes through the spatial mask to the 4f system; an optical granting, disposed on a Fourier plane in middle of the 4f system, and configured to split the real image of the sample to obtain a split real image; and an image sensor, configured to obtain the split real image to obtain an image of the sample. The present disclosure advantages of improving imaging rate in multi-spectrum fluorescence microscopy.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.