Method for preparing foot-and-mouth disease virus-like particles

US10829741B2 · kind B2 · utility

Assignee

• LANZHOU VETERINARY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Shiqi SUN · Lanzhou, CN
• Huichen GUO · Lanzhou, CN
• Yun ZHANG · Lanzhou, CN
• Yanyan CHANG · Lanzhou, CN
• Yanquan WEI · Lanzhou, CN
• Jiaxi Ru · Lanzhou, CN
• Xiaoying ZHI · Lanzhou, CN
• Ping Du · Rahway, US
• Xiangtao Liu · Lanzhou, CN
• Hong Yin · Hangzhou City, CN
• Jianxun LUO · Lanzhou, CN

Key dates

• Filing date: May 31, 2018
• Grant date: Nov 10, 2020
• Expiry date: Jun 12, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/26
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for preparing serotype O foot-and-mouth disease virus-like particles, the method including: construction of small ubiquitin-like modifier fusion expression vector, construction of recombinant expression vectors, construction of recombinant co-expression vector, expression and purification of proteins, and in-vitro assembly of serotype O foot-and-mouth disease virus-like particles. The disclosure also provides a test strip for detecting serotype O foot-and-mouth disease including a bottom board, and a detection layer disposed on the top of the bottom board. A detection line and a control line are disposed on the detection layer. An absorbent layer is disposed at one end of the detection layer close to the control line, and an immuno-gold pad is disposed at the other side of the detection layer close to the detection line. A sample pad is disposed on the top of the immuno-gold pad.