Method for preparing recombinant adeno-associated virus

US10829784B2 · kind B2 · utility

Assignee

• WUHAN INSTITUTE OF PHYSICS AND MATHEMATICS, CHINESE ACADEMY OF SCIENCES · CN

Inventors

• Yang-Chang Wu
• Fuqiang XU · Shanghai, CN
• Xiaobin He · Los Angeles, US
• Kunzhang Lin · 红钢城街道, CN

Key dates

• Filing date: Jan 30, 2018
• Grant date: Nov 10, 2020
• Expiry date: Nov 19, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for producing recombinant adeno-associated virus, the method including: (1) transforming a gene of interest (GOI) into a recombinant baculovirus, where the recombinant baculovirus has a genome integrated with AAV Rep gene, Cap gene, and rAAV genome ITR-GOI that are needed in the production of the rAAV; and where the ITR-GOI is linked to expression cassette of the Cap gene and the Rep gene by a 5′ terminal nucleic acid segment or a 3′ terminal nucleic acid segment; (2) infecting host insect larvae with the recombinant baculovirus prepared in (1), such that the rAAV is produced in vivo in the host insect larvae; and (3) lysing the host insect larvae obtained in (2), and extracting and purifying the rAAV.