Method for identification and enumeration of nucleic acid sequences, expression, splice variant, translocation, copy, or DNA methylation changes using combined nuclease, ligase, polymerase, terminal transferase, and sequencing reactions

US10829804B2 · kind B2 · utility

Assignees

• The University of North Carolina at Chapel Hill · US
• Cornell University · IT
• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US

Inventors

• Francis Barany · Baltimore, US
• John William Efcavitch · San Carlos, US
• Steven A. Soper · Baton Rouge, US
• Sunggook Park · Baton Rouge, US

Key dates

• Filing date: Mar 23, 2016
• Grant date: Nov 10, 2020
• Expiry date: Mar 27, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is directed methods for identifying, in a sample, one or more target nucleotide sequences differing from other nucleotide sequences in the sample by one or more nucleotides, one or more copy numbers, one or more transcript sequences, and/or one or more methylated residues, using ligation detection reactions, polymerase mediated extension reactions, and/or cleavage reactions. The present invention is also directed to methods for identifying, in a sample, one or more nucleotides in a target nucleotide sequence.