Universal molecular processor for precision medicine

US10830757B2 · kind B2 · utility

Assignees

• The University of North Carolina at Chapel Hill · US
• Cornell University · IT
• BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE · US

Inventors

• Steven A. Soper · Baton Rouge, US
• Francis Barany · Baltimore, US
• Sunggook Park · Baton Rouge, US
• Michael C. Murphy · Baton Rouge, US
• Collin J. McKinney · Durham, US
• John William Efcavitch · San Carlos, US
• Mateusz Hupert · Lawrence, US

Key dates

• Filing date: Jul 17, 2019
• Grant date: Nov 10, 2020
• Expiry date: Jul 17, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention is directed to methods comprising a device that comprises a biomolecular processor and one or more nanotubes. Each biomolecular processor comprises a bioreactor chamber defined by a solid substrate, a plurality of spaced support structures within said bioreactor chamber and attached to the solid substrate, one or more nanotubes defined by the solid substrate and fluidically coupled to the bioreactor chamber and one or more capture molecules immobilized to some or all of said plurality of spaced support structures, said one or more capture molecules suitable to bind to a portion of a target nucleic acid molecule in a sample. The nanotubes have a passage extending between an input end proximate to the bioreactor chamber and an output end distal to the bioreactor chamber, and comprises one or more nanopores within the passage with each nanopore having a reduced diameter relative to the passage.