Markers of papillary and reticular fibroblasts and uses thereof
US10837058B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 23, 2012 |
| Grant date | Nov 17, 2020 |
| Priority date | — |
| Expiry date | Jun 29, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/7042
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An in vitro method for screening for candidate compounds for preventing and/or attenuating skin ageing, and/or hydrating skin, includes: a) contacting a test compound with a sample of papillary fibroblasts; b) measuring the expression of a gene selected from PDPN, CCRL1 and NTN1, in the papillary fibroblasts; and c) selecting compounds for which an activation of at least 1.5 fold of the expression of at least one of the genes is measured in the treated papillary fibroblasts compared with untreated papillary fibroblasts. Another in vitro method includes: a) contacting a test compound with a sample of reticular fibroblasts; b) measuring the expression of a gene selected from MGP, PPP1R14A and TGM2, in the reticular fibroblasts; and c) selecting compounds for which an activation of at most 1.0 fold of the expression of at least one of the genes is measured in the treated reticular fibroblasts compared with untreated reticular fibroblasts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.