Rice mitochondrial sterile gene and application thereof

US10837068B2 · kind B2 · utility

Assignees

• Jiangxi Super-rice Research and Development Center · CN
• Nanchang University · CN

Inventors

• Yaohui Cai · Nanchang, CN
• Hongwei Xie · Nanchang, CN
• Xiaojue Peng · Nanchang, CN
• Mingjuan Qian · Nanchang, CN
• Yicong Cai · Nanchang, CN
• Xia Ding · 安丰镇, CN
• Youlin Zhu · Nanchang, CN
• Long'An Yan · Nanchang, CN

Key dates

• Filing date: Jan 22, 2018
• Grant date: Nov 17, 2020
• Expiry date: Feb 26, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/13
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a rice mitochondrial sterile gene and the application thereof. A rice mitochondrial sterile gene and a coding sequence thereof are disclosed. A specific molecular marker was designed from said gene sequence and enables a rapid and effective screening and identification of D1-type cytoplasm from wild rice for breeding a new D1-type cytoplasm sterile line. A specific sequence in the mitochondria genome of D1-type cytoplasm sterile line was identified by comparative genomics. Sterility-related ORFs were identified from gene prediction and expression difference analysis. A plant transformation vector was constructed to transform maintainer line for verification and sterile function.