Method for detecting total 25-hydroxyvitamin D using antibodies to 25-hydroxyvitamin D2 and D3
US10837973B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 7, 2018 |
| Grant date | Nov 17, 2020 |
| Priority date | — |
| Expiry date | Aug 2, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided herein are antigenic molecules that can be used to generate antibodies capable of binding to a vitamin D derivative, such as 25-hydroxyvitamin D2 and/or 25-hydroxyvitamin D3, or a 25-hydroxyvitamin D analog, such as a vitamin D-C22 immunogenic molecule or compound. Antibodies produced using these antigenic molecules, and related antigenic compounds, are also described. In addition, disclosed herein are methods for detecting vitamin D deficiency in a subject, methods for treating a subject suspected of having a vitamin D deficiency, methods for monitoring progression of vitamin D deficiency in a subject, and methods for monitoring treatment of vitamin D deficiency in a subject in need thereof. The methods involve the detection or quantification of 25-hydroxyvitamin D2 and D3. Also provided are methods and reagents for the detection or quantification of 25-hydroxyvitamin D2 and D3, methods for stabilizing vitamin D analogs, and methods for separating 25-hydroxyvitamin D2 and D3 from vitamin D binding protein in a biological sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.