Polypeptide having the capacity to form alpha-1,3 glucosyl unit branchings on an acceptor

US10842808B2 · kind B2 · utility

Assignees

• INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION L'ENVIRONNEMENT · FR
• INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE · FR
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR

Inventors

• Magali Remaud-Simeon · Ramonville-Saint-Agne, FR
• Marlene Vuillemin · Søborg, DK
• Claire Moulis · Saint-Jean, FR
• Pierre Monsan · Mondonville, FR
• Sandrine Morel · Ramonville-Saint-Agne, FR

Key dates

• Filing date: Sep 22, 2017
• Grant date: Nov 24, 2020
• Expiry date: Oct 6, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y204/01
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.