Method for preparing bacterial polysaccharide-modified recombinant fusion protein and use of the protein

US10844098B2 · kind B2 · utility

Assignee

• Institute of Biotechnology, Academy of Military Medical Sciences, China · CN

Inventors

• Jun Wu · Beijing, CN
• Chao-Chi Pan · Hsinchu, TW
• Peng Sun · Beijing, CN
• Hengliang Wang · Beijing, CN
• Bo Liu · Beijing, CN
• Zhehui Peng · Beijing, CN
• Li Zhu · Burbank, US
• Shaohong Chang · Beijing, CN
• Xin Gong · Qingdao, CN
• Erling Feng · Beijing, CN
• Bin Wang · Beijing, CN
• Ming Zeng · Beijing, CN

Key dates

• Filing date: Sep 1, 2015
• Grant date: Nov 24, 2020
• Expiry date: Sep 25, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y204/01
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.