FAD2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

US10844389B2 · kind B2 · utility

Assignees

• DOW AGROSCIENCES LLC · US
• Sangamo Therapeutics, Inc. · US

Inventors

• W. Michael Ainley · Carmel, US
• Steven R. Webb · Westfield, US
• Jayakumar P. Samuel · Carmel, US
• Dmitry Y. Guschin · Seojong-myeon, KR
• Jeffrey C. Miller · Pleasanton, US
• Lei Zhang · Beijing, CN

Key dates

• Filing date: Apr 23, 2019
• Grant date: Nov 24, 2020
• Expiry date: Apr 23, 2039

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.