Spinosad heterologous expression strain and construction method thereof and use

US10844415B2 · kind B2 · utility

Assignee

• Zhejiang Hisun Pharmaceutical Co., Ltd. · CN

Inventors

• Jun Huang · Lo Wu, CN
• Zhen Yu · 流花街道, CN
• Meihong Li · Qiaonanxiang, CN
• Linghui Zheng · Shanghai, CN
• Na Li · Beijing, CN
• Haibin Wang · Beijing, CN
• Hua Bai · Lake Jackson, US

Key dates

• Filing date: Nov 14, 2014
• Grant date: Nov 24, 2020
• Expiry date: Nov 14, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/74
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a construction method of spinosad heterologous expression strain, a spinosad heterologous expression strain obtained by the method and use thereof in preparing spinosad. The method utilizes a plurality of homologous recombination to replace the erythromycin synthetic gene cluster of Saccharopolyspora erythraea with the spinosad synthetic gene cluster and the rhamnose synthetic gene cluster, such that the Saccharopolyspora erythraea produces spinosad.