Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)

US10844428B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Sante Gnerre · Mountain View, US
• Byoungsok Jung · Palo Alto, US
• Emrah Kostem · Redwood City, US
• Alex Aravanis · San Francisco, US
• Alex So · San Diego, US
• Xuyu Cai · Natick, US
• Zhihong Zhang · Chandler, US
• Frank J. Steemers · Encinitas, US

Key dates

• Filing date: Apr 15, 2016
• Grant date: Nov 24, 2020
• Expiry date: Apr 15, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B30/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosed embodiments concern methods, apparatus, systems and computer program products for determining sequences of interest using unique molecular index (UMI) sequences that are uniquely associable with individual polynucleotide fragments, including sequences with low allele frequencies and long sequence length. In some implementations, the UMIs include both physical UMIs and virtual UMIs. In some implementations, the unique molecular index sequences include non-random sequences. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.