DNA sequencing reaction additive

US10844430B2 · kind B2 · utility

Assignee

• QIAGEN Sciences, LLC · US

Inventors

• Luisa Andruzzi · Beverly, US
• Jimmy Adediran · Waltham, US
• Timothy Pelletier · Leominster, US
• Austin Ricker · Waltham, US
• Angela DeLucia · Cambridge, US
• Ben McNally · Medford, US
• Dona Hevroni · Lexington, US
• Mong Sano Marma · Natick, US
• John Andrew Sheridan · Marblehead, US

Key dates

• Filing date: Jan 24, 2018
• Grant date: Nov 24, 2020
• Expiry date: May 31, 2038

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides methods, compositions, mixtures and kits utilizing 5-Chloro-2-methyl-4-isothiazolin-3-one in sequencing reactions, and in particular, sequencing reactions where deoxynucleoside triphosphates comprising a 3′-O position capped by a disulfide-based 3′-terminator group are used. In one embodiment, the deoxynucleoside triphosphates comprise a 3′-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. In addition, thiol-containing compounds and scavengers of thio-containing compounds are described. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.