Mycobacterium neoaurum-derived steroid C27-monooxygenase and application thereof
US10851352B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 29, 2016 |
| Grant date | Dec 1, 2020 |
| Priority date | — |
| Expiry date | Jan 18, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/13072
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a Mycobacterium neoaurum-derived steroid C27-monooxygenase and an application thereof, which belong to the technical fields of genetic engineering and enzyme engineering. By the method of gene knockout and intensive expression, the present invention screens out three isoenzymes of a key enzyme SMO in the process of degrading sterol side chains from Mycobacterium neoaurum. The three isoenzymes are intensively expressed respectively in the Mycobacterium neoaurum for the high yield of androsta-1,4-diene-3,17-dione (ADD), the yield of ADD is increased remarkably, wherein the effect of SMO2 is most remarkable. By overexpressing SMO2, the final ADD yield is increased from 5.2 g·L−1 to 7.3 g·L−1. The present invention provides a helpful guidance for the industrialization of the microbial fermentation method for increasing the ADD yield.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.