Nucleic acid sequencing using affinity reagents

US10851410B2 · kind B2 · utility

Assignees

• MGI Tech Co., Ltd. · CN
• BGI SHENZHEN · CN

Inventors

• Radoje Drmanac · Los Altos Hills, US
• Snezana Drmanac · Nanhu, CN
• Handong Li · San Jose, US
• Xun Xu · Shanghai, CN
• Matthew J. Callow · Redwood City, US
• Leon Eckhardt · Soquel, US
• Naibo Yang · San Jose, US

Key dates

• Filing date: Jan 4, 2018
• Grant date: Dec 1, 2020
• Expiry date: Jun 13, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/73
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.