Method and device for acquiring and displaying an immunofluorescence image of a biological sample

US10855923B2 · kind B2 · utility

Assignee

• EUROIMMUN Medizinische Labordiagnostika AG · DE

Inventors

• Tilman Johannes Sumpf · Hamdorferweide, DE
• Michael Pape · Stade, DE
• Martin Hagen-Eggert · Stade, DE
• Christian Piepke · Stade, DE
• Thomas Laudan · Kiel, DE
• Michael Falkert · Reinfeld, DE
• Markus Morrin · Carlow, DE
• Kristin Rentzsch · Bad Schwartau, DE

Key dates

• Filing date: Sep 13, 2019
• Grant date: Dec 1, 2020
• Expiry date: Sep 13, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method is used for acquiring and displaying an immunofluorescence image of a biological sample. During a first operating state, the sample is continuously illuminated using the excitation radiation. A relative position between the sample and an optical system, which guides the fluorescent radiation to an image sensor, is changed in dependence on a movement gesture of a user, and a corresponding digital image is displayed. Upon detection of a termination of the movement gesture, a change is made from the first operating state to a second operating state. In the second operating state, the sample is firstly illuminated using the excitation radiation to excite the fluorescent radiation of the fluorescent pigment. An acquisition of fluorescent radiation emitted by the sample then takes place and a corresponding, further digital image is generated. In the second operating state, the acquisition of the emitted fluorescent radiation and the illumination of the sample are terminated after lapse of the second acquisition duration.