Compositions and methods for reprogramming non-neuronal cells into neuron-like cells

US10857185B2 · kind B2 · utility

Assignees

• BeiHao Stem Cell and Regenerative Medicine Research Institute Co., Ltd. · CN
• Peking University · CN

Inventors

• Hongkui Deng · Beijing, CN
• Xiang Li · Beijing, CN
• Xiaohan Zuo · Beijing, CN

Key dates

• Filing date: Jul 1, 2016
• Grant date: Dec 8, 2020
• Expiry date: Nov 28, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2506/1392
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Cocktails of chemical inducers of neuron-like properties (CINP) is provided, which includes cAMP agonists, neurogenic small molecules, glycogen synthase kinase inhibitors, TGFβ receptor inhibitors, and BET family bromodomain inhibitors and optionally, a selective inhibitor of ROCK or p38 MAPK. These cocktails are used in a method of inducing neuron-like properties in partially or completely differentiated non-neuronal cells. The method includes contacting cells of a first type (non-neuronal) with the CINPs for a sufficient period of time to result in reprogramming the cell into cells of a second type having neuron-like characteristics (CiNs). Isolated chemically induced neurons (CiNs) can be used in a number of applications, including but not limited to cell therapy.