Techniques for predicting, detecting and reducing aspecific protein interference in assays involving immunoglobulin single variable domains

US10858418B2 · kind B2 · utility

Assignee

• Ablynx N.V. · BE

Inventors

• Judith Baumeister · Wuppertal, DE
• Marie-Paule Lucienne Armanda Bouche · Gentbrugge, BE
• Carlo Boutton · Wielsbeke, BE
• Marie-Ange Buyse · Merelbeke, BE
• Veerle Snoeck · Zingem, BE
• Stephanie Staelens · Wevelgem, BE
• Bruno Dombrecht · Heusden, BE
• Peter Schotte · Zevergem, BE
• Cedric Jozef Neotere Ververken · Merelbeke, BE
• Gerald Beste · Evergem, BE
• Guy Hermans · Merelbeke, BE
• Soren Steffensen · Woluwe-Saint-Pierre - Sint-Pieters-Woluwe, BE
• Alexander Szyroki · Goldenstedt, DE
• Tinneke Denayer · Zevergem, BE

Key dates

• Filing date: Mar 18, 2020
• Grant date: Dec 8, 2020
• Expiry date: Mar 18, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6857
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention provides, and in certain specific but non-limiting aspects relates to: assays that can be used to predict whether a given ISV will be subject to protein interference as described herein and/or give rise to an (aspecific) signal in such an assay (such as for example in an ADA immunoassay). Such predictive assays could for example be used to test whether a given ISV could have a tendency to give rise to such protein interference and/or such a signal; to select ISV's that are not or less prone to such protein interference or to giving such a signal; as an assay or test that can be used to test whether certain modification(s) to an ISV will (fully or partially) reduce its tendency to give rise to such interference or such a signal; and/or as an assay or test that can be used to guide modification or improvement of an ISV so as to reduce its tendency to give rise to such protein interference or signal; —methods for modifying and/or improving ISV's to as to remove or reduce their tendency to give rise to such protein interference or such a signal; —modifications that can be introduced into an ISV that remove or reduce its tendency to give rise to such protein interference …