Production for recombinant human DNase I
US10858640B2 · kind B2 · utility
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12Claims
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Key dates
| Filing date | Apr 28, 2017 |
| Grant date | Dec 8, 2020 |
| Priority date | — |
| Expiry date | Apr 28, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/09
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for production of recombinant human DNase I which is of such high purity as may be directly used as a medical drug. The method includes the steps of; culturing recombinant DNase I-producing mammalian cells, subjecting a culture supernatant to an anion-exchange column chromatography, subjecting to a column chromatography employing as solid phase a material having affinity for phosphate group, subjecting to a cation-exchange column chromatography, and subjecting to a dye affinity column chromatography.
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