Thermostable reverse transcriptase

US10858652B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Andrew Ellington · Austin, US
• Jared Ellefson · Austin, US
• Jimmy Gollihar · Austin, US

Key dates

• Filing date: May 8, 2019
• Grant date: Dec 8, 2020
• Expiry date: Jun 6, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/447
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.