Nucleic acid amplification

US10858695B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Chieh-Yuan LI · Hayward, US
• David Ruff · North Hinksey, GB
• Jennifer O'Neil · Peabody, US
• Rachel Kasinskas · Amesbury, US
• Shiaw-Min Chen · San Jose, US
• Jonathan M. Rothberg · Paradise, US
• Bin Li · Columbus, US
• Kai Qin Lao · Pleasanton, US

Key dates

• Filing date: Oct 23, 2018
• Grant date: Dec 8, 2020
• Expiry date: Jul 7, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In some embodiments, provided are methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.