Patent · US Active

Cleavable hairpin primers

US10858699B2 · kind B2 · utility

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9Claims
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Key dates

Filing dateAug 29, 2017
Grant dateDec 8, 2020
Priority date—
Expiry dateOct 11, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2310/122
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention describes composition and methods of use for novel hairpin blocked-cleavable primers. In one embodiment unblocking occurs through action of RNase H2. The method improves the specificity of PCR and reduces primer dimer events, enabling higher level multiplex reactions. Additionally, the invention protects RNA-containing primers from attack by single-strand RNases.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.