Patent · US Active

Compositions and methods for improving nanopore sequencing

US10858700B2 · kind B2 · utility

1Cited by

5References

17Claims

0Family size

Assignee

University of Washington through its Center for Commercialization · US

Inventors

Jens H. Gundlach · Seattle, US
Andrew Laszlo · Seattle, US

Key dates

Filing date	Aug 2, 2013
Grant date	Dec 8, 2020
Priority date	—
Expiry date	Apr 16, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6869
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present disclosure provides methods and reagents for improving nanopore-based analyses of polymers. Specifically, the disclosure provides a method of analyzing a polymer that includes a polymer analyte that contains an end domain that has at least one charged moiety. The disclosure also provides a method of increasing the interaction rate between a polymer analyte and a nanopore, wherein the polymer analyte contains an end domain that has at least one charged moiety. The disclosure also provide compositions for use with the described methods, including adapter compositions that contain charged moieties, such as phosphate or sulfate groups, and that are configured to being linked to an polymer analyte domain.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.