Processing cartridge and method for detecting a pathogen in a sample

US10864522B2 · kind B2 · utility

Assignee

• GenMark Diagnostics, Inc. · US

Inventors

• Scott Corey · Hydes, US
• Brian Murphy · Baltimore, US
• Eric Schneider · Catonsville, US
• David W. Wright · Littleton, US
• Dominic Aiello · Denver, US
• Andrew Fish · Carlsbad, US
• Jay Srinivasan · San Francisco, US
• George Maltezos · Fort Salonga, US

Key dates

• Filing date: Aug 15, 2019
• Grant date: Dec 15, 2020
• Expiry date: Aug 15, 2039

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/06
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.