Engineered alanyl-glutamine dipeptide biosynthetic enzyme and application thereof
US10865225B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 27, 2019 |
| Grant date | Dec 15, 2020 |
| Priority date | — |
| Expiry date | Jun 27, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a gene encoding alanyl-glutamine dipeptide biosynthetic enzyme and the application thereof. The nucleotide sequence of said gene is shown in SEQ ID NO: 1. Further, provided is an amino acid sequence encoded by the gene, and provided recombinant vector, recombinant E. coli, recombinant S. cerevisiae, and a self-flocculating recombinant S. cerevisiae containing the gene. Furthermore, provided is a method for carrying out bioconversion to synthesize alanyl-glutamine dipeptide by using said recombinant microorganisms. The application of the gene and recombinant batteries has the characteristics of high molar conversion rate, fast production rate and repeated recycling of the bacteria. It is a good choice for efficient, environmentally friendly and economical production of alanyl-glutamine dipeptide. In addition, through the recycling process, it is expected to reduce the cost of industrial production, solve the problems of difficult enzyme recovery, low utilization rate and unstable enzyme activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.