Method for improving the culture conditions of cultures of primary sweat gland cells and/or three-dimensional sweat gland equivalents

US10865380B2 · kind B2 · utility

Assignee

• Henkel AG & Co. KGaA · DE

Inventors

• Patricia Klaka · Leverkusen, DE
• Bernhard Banowski · Goldkronach, DE
• Sabine Gruedl · Erkelenz, DE
• Thomas Welss · Dressendorf, DE

Key dates

• Filing date: Dec 7, 2017
• Grant date: Dec 15, 2020
• Expiry date: Feb 9, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2513/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to a method for improving the culture conditions of cultures of primary sweat gland cells and/or three-dimensional sweat gland equivalents, where the culture with from about 500 to about 500,000 sweat gland cells and a diameter of from about 100 to about 6,000 nm is initially prepared and then cultivated in a culture medium with neurotransmitters and/or from about 11 to about 100 ng/ml of at least one growth factor. Cultivation of these cells and models in a culture medium containing at least one neurotransmitter and/or from about 11 to about 100 ng/ml of at least one growth factor leads to an increase of sweat-gland-specific marker proteins. As a result, a loss of function, which occurs with an absence of or only low expression of these markers, can be avoided.