Aspartase mutant, recombinant expression vector and recombinant bacterium containing aspartase mutant, and use thereof

US10865404B1 · kind B1 · utility

Assignee

• Jiangnan University · CN

Inventors

• Zhiming Rao · Wuxi, CN
• Yaling Wang · Westfield, US
• Jiamin Chen · 安丰镇, CN
• Taowei Yang · Wuxi, CN
• Meijuan Xu · Wuxi, CN
• Xian Zhang · Beijing, CN
• Minglong Shao · Wuxi, CN
• Jianing Zhang · Beijing, CN
• Anqi Peng · 安丰镇, CN
• Shuping Xu · 安丰镇, CN
• Meiqi Wu · 安丰镇, CN

Key dates

• Filing date: Jun 15, 2020
• Grant date: Dec 15, 2020
• Expiry date: Jun 15, 2040

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y403/01001
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides an aspartase mutant, a recombinant expression vector and recombinant bacterium containing the aspartase mutant, and the use thereof, and belongs to the technical field of genetic engineering. The amino acid sequence of the aspartase mutant is as set forth in SEQ ID NO: 1. In the aspartase mutant of the present invention, on the basis of wild type aspartase (with an amino acid sequence as set out in SEQ ID NO: 3), glutamic acid at position 427 is mutated into glutamine. In the present invention, by mutating the amino acid residue at position 427 into glutamine, the polar environment near an active site is changed, and thus ammonia supply during substrate reaction is further facilitated, thereby improving an enzyme activity, enhancing the ability of the enzyme in synthesizing a β-amino acid, and providing a practical and effective strategy for industrial production of the β-amino acid.