Method of screening antibacterial compounds as inhibitor of Mfd
US10865435B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 3, 2017 |
| Grant date | Dec 15, 2020 |
| Priority date | — |
| Expiry date | Jul 10, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16C20/60
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Embodiments of the present disclosure relate to methods of screening and optionally designing compounds as inhibitors of Mfd (Mutation Frequency Decline), such compounds being useful for the treatment of bacterial infections. Embodiments of the methods include culturing pathogenic bacteria expressing functional Mfd with various concentrations of 1 mM or less, incubating the cultures with and without nitric oxide stress conditions, evaluating bacterial survival in each of the cultures, and calculating the concentration of the molecule required to decrease by 50% the survival rate of the bacteria (IC50) by comparing the cfu of the untreated cultures and cultures following nitric oxide stress.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.