Devices and methods for direct visual detection and readout of single nucleic acid molecules
US10866184B2 · kind B2 · utility
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28Claims
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Key dates
| Filing date | Nov 4, 2016 |
| Grant date | Dec 15, 2020 |
| Priority date | — |
| Expiry date | Dec 17, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to methods, computer readable medium and systems for detecting and counting single nucleic acid molecules confined in nanoliter volumes using an unmodified camera, such as a cell phone camera. In particular, it identifies colorimetric amplification-indicator dyes that are compatible with the spectral sensitivity of standard mobile phones. The invention further provides an optimal ratiometric image-process for a selected dye to achieve a readout that is robust to lighting conditions and camera hardware and provides unambiguous quantitative results, even for colorblind users.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.