Cellular high throughput encapsulation for screening or selection
US10870846B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 9, 2013 |
| Grant date | Dec 22, 2020 |
| Priority date | — |
| Expiry date | May 29, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1068
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for selecting a sequence set from a library of expressed nucleic acid sequences, wherein cells are provided, each cell comprises an expressed nucleic acid sequence expressed as a target protein. The cells are encapsulated by treating them with a cationic polysaccharide and subsequently treating them with an anionic polysaccharide, yielding encapsulated cells, perforating the membrane of the encapsulated cells, yielding solubilized compartments, contacting them with a ligand to said target protein, the ligand bearing a detectable label, and selecting a subset of solubilized compartments as a function of detectable label and isolating the expressed nucleic acid sequences from the selection as a selected sequence set.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.